Tes
t concentrations in runs two and three (if applicable) should be adjusted on the basis of the results of the initial run bracke
ting concentrations that elicited an effect using 1/2-log concentration spacing (e.g. if the original run of 0,001, 0,01, 0,1, 1, 10, 100, 1 000 μM resulted in inductions at 1 and 10 μM, the concentrations tested in the second run should be 0,1, 0,3, 1, 3, 10, 30, 100 μM), unless l
ower concentrations need to be employed to a ...[+++]chieve a LOEC.